Enosi

Summary

Our proteogenomics software has two distinct toolkits. The first involves the creation of specialized databases that can capture variant gene events, and is available in the SpliceDB tool . These customized databases can be searched against tandem mass spectra using any tool, but we recommend MS-GF+. Finally, the Enosi pipeline analyzes the identified peptides. Enosi is developed to automize the spectrum-peptide match to be recognized more intuitive sense. Because peptide sequence itself are not very informative for novel peptides, Enosi classifies the peptides as known and novel and finds the genomic locus for the novel. And it compares these novel locations with known gene sets and classify them into event to make a novel peptide to be more intuitively recognizable. It also contain its own method to filtering out some uncredible events.

• Input: Output of MS-GF+ or other search tool
• Output: List of event. Each event contains following information – Genomic location, Related known gene, Supporting RNA information, UCSC genome browser link, number of spectrum, number of novel and known peptides supporting current event, etc.
• Event List: Alternative Splice, Novel Splice, Fusion Gene, Insertion, Deletion, Mutation, Translated UTR, Gene boundary, Exon boundary, Novel exon, Frame shift, Reverse strand, Novel gene